1. Log in into Metabolizer. For further information on this step visit Logging in.

2. We will work with a Breast Cancer dataset from the repository The Cancer Genome Atlas. You can download the expression matrix and the experimental design from these links:

• Expression matrix: brca_example1_40_exp.txt
• Experimental design: brca_example1_40_design.txt

3. Upload the data to Metabolizer in the data panel by clicking on My data. For further information on this step visit Upload your data.

4. Press the Prediction button.

5. In the Type panel select Train new model.

6. In the Input data panel select Expression matrix. Press the File browser of the Expression matrix file, and select the desired file.

7. Press the File browser of the Experimental design file, and select the desired file. All conditions in your design file will be shown on the panel. You do not need to do a selection.

8. Select a machine learning that you want to use.

9. Select Human as species (by default).

10. In the Job information panel, press the File browser button and select the desired output folder. In this case we will use analysis. Give a name to the job, for example “BRCA train model”.

11. Press the Launch job button. A job will be created and listed in the jobs panel. You can access this panel by clicking on the My jobs button.