1. Log in into Metabolizer. For further information on this step visit Logging in.

2. We will test the model with another Breast Cancer dataset from the repository The Cancer Genome Atlas. You can download the expression matrix of the test data (one of them) from the link:

• Test expression matrix1: brca_example1_40_exp.txt (this is the same dataset used for training)
• Test expression matrix2: brca_2_sample_test.txt (contains 2 new samples, 1 tumor and 1 normal)

3. Upload the data to Metabolizer in the data panel by clicking on My data. For further information on this step visit Upload your data.

4. Press the Prediction button.

5. In the Type panel, select Test existing model. A window with all the existing models will appear. Select the model you want to use. We will use the model we have created in Worked example Prediction - Train. The model information will appear on the right panel.

6. In the Input data panel select Expression matrix. Press the File browser of the Expression matrix file, and select the desired file, brca_example1_40_exp.txt.

7. In the Job information panel, press the File browser button and select the desired output folder. In this case we will use analysis. Give a name to the job, for example “BRCA test model”.

8. Press the Launch job button. A job will be created and listed in the jobs panel. You can access this panel by clicking on the My jobs button.