1- Log in into Metabolizer. For further information on this step visit Logging in.

2- We will work with a Breast Cancer dataset from the repository The Cancer Genome Atlas. You can download the expression matrix and the experimental design from these links:

• Expression matrix: brca_example1_40_exp.txt
• Experimental design: brca_example1_40_design.txt

3- Upload the data to Metabolizer in the data panel by clicking on My data. For further information on this step visit Upload your data.

4- Press the Activity button.

5- Press the File browser of the Expression matrix file, and select the desired file.

6- Press the File browser of the Experimental design file, and select the desired file. Automatically Condition 1 and Condition 2 files are selected. Change Condition 1 to “Tumor” and Condition 2 to “Normal”.

7- Select Human as species.

8- In the Job information panel, press the File browser button and select the desired output folder. Give a name to the job, for example “BRCA module activity”.

11- Press the Launch job button. A job will be created and listed in the jobs panel. You can access this panel by clicking on the My jobs button.