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worked_example_differential_example [2016/12/26 12:43] ccubukworked_example_differential_example [2017/05/24 14:33] (current) – external edit 127.0.0.1
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-====== Worked example Differential signaling ======+====== Worked example Differential Module Activity ======
  
 **1-** Log in into Metabolizer. For further information on this step visit [[logging_in|Logging in]]. **1-** Log in into Metabolizer. For further information on this step visit [[logging_in|Logging in]].
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 **2-** We will work with a Breast Cancer dataset from the repository The Cancer Genome Atlas. You can download the expression matrix and the experimental design from these links: **2-** We will work with a Breast Cancer dataset from the repository The Cancer Genome Atlas. You can download the expression matrix and the experimental design from these links:
  
-  * Expression matrix: {{:brca_genes_vals_bn.txt|}} + 
-  * Experimental design: {{:brca_normal-basal_ed.txt|}}+  * Expression matrix: {{::brca_example1_40_exp.txt|}} 
 +  * Experimental design: {{::brca_example1_40_design.txt|}}
  
 **3-** Upload the data to Metabolizer in the data panel by clicking on //My data//. For further information on this step visit [[upload_your_data|Upload your data]]. **3-** Upload the data to Metabolizer in the data panel by clicking on //My data//. For further information on this step visit [[upload_your_data|Upload your data]].
  
 **4-** Press the //Activity// button.  **4-** Press the //Activity// button. 
- 
-{{ :hipathia_bar_diffsig.png?600 |}} 
  
 **5-** Press the //File browser// of the //Expression matrix file//, and select the desired file. **5-** Press the //File browser// of the //Expression matrix file//, and select the desired file.
  
-{{ :hipathia_work1.png?600 |}} 
  
 **6-** Press the //File browser// of the //Experimental design file//, and select the desired file. Automatically //Condition 1// and //Condition 2// files are selected. Change //Condition 1// to "Tumor" and //Condition 2// to "Normal". **6-** Press the //File browser// of the //Experimental design file//, and select the desired file. Automatically //Condition 1// and //Condition 2// files are selected. Change //Condition 1// to "Tumor" and //Condition 2// to "Normal".
- 
-{{ :hipathia_work2.png?600 |}} 
  
 **7-** Select Human as species. **7-** Select Human as species.
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 **8-** In the //Job information// panel, press the //File browser// button and select the desired output folder. Give a name to the job, for example "BRCA module activity". **8-** In the //Job information// panel, press the //File browser// button and select the desired output folder. Give a name to the job, for example "BRCA module activity".
  
-{{ :hipathia_work3.png?600 |}} 
  
 **11-** Press the //Launch job// button. A job will be created and listed in the jobs panel. You can access this panel by clicking on the //My jobs// button. **11-** Press the //Launch job// button. A job will be created and listed in the jobs panel. You can access this panel by clicking on the //My jobs// button.
worked_example_differential_example.1482756230.txt.gz · Last modified: 2017/05/24 14:33 (external edit)